Product Description:
The principle of fecal occult blood test mainly relies on chemical reaction to detect occult blood in stool samples. The following is a detailed test principle:
### **Principle of fecal occult blood test**
#### **1. Basic principle of occult blood test**
Fecal occult blood test is designed to detect trace amounts of hemoglobin or hemoglobin degradation products in stool samples. This test usually uses immunochemical methods or chemical reaction methods to identify potential blood components.
#### **2. Chemical reaction method**
**Calatase reaction**:
- **Reaction mechanism**: Some fecal occult blood test kits use catalase (such as catalase in bovine hemoglobin) and the principle of redox reaction. The kit contains a chemical substance (such as a reagent for false positive reaction) that reacts with occult blood in the stool sample, which undergoes a redox reaction and produces a color change.
- **Steps**:
1. Apply the stool sample to the test strip or test card.
2. Add reagents, usually including a hydroperoxide and a dye.
3. If occult blood is present in the sample, the catalase in hemoglobin will catalyze the reagent reaction, resulting in color development.
4. Based on the intensity and appearance of the color change, determine whether the sample contains occult blood.
**Color reaction**:
- **Color reagent**: Common color reagents in the kit include hydrogen peroxide and specific dyes such as cyanine (for a reagent called pseudoamino acid-salicylate reaction).
- **Reaction process**: Catalase in hemoglobin catalyzes the oxidation reaction of these dyes, producing a significant color change. The intensity and appearance of the color are proportional to the amount of occult blood.
#### **3. Immunochemical method**
**Immunochemical method**:
- **Principle**: Some fecal occult blood kits use the immunochemical principle, that is, antibody-antigen reaction to detect blood components in stool. The method is based on specific antibodies to hemoglobin or its decomposition products.
- **Steps**:
1. Process the stool sample and apply it to the reagent card.
2. Add labeled antibodies that specifically bind to hemoglobin in the sample.
3. If hemoglobin is present in the sample, the antibodies bind to it and produce a color change or signal through the attached marker.
4. Observe the signal or color change on the test card to confirm the result.
#### **4. Result Interpretation**
- **Negative result**: The test area does not develop color or the color line is weak, which usually means that not enough occult blood is detected in the stool sample.
- **Positive result**: The test area develops color or the color line is obvious, indicating that occult blood is present in the sample and further medical examination is required.
- **Invalid result**: If the control line of the kit does not develop color or the reagent card shows abnormality, it may be necessary to retest or check the quality of the kit.
#### **5. Precautions**
- **Food and drug effects**: Certain foods (such as red meat) or drugs (such as anti-inflammatory drugs) may interfere with the test results. Therefore, it is recommended to follow relevant dietary and drug taboos before taking the test.
- **Operation Specifications**: Make sure to follow the instructions to avoid sample contamination and operation errors.
Fecal occult blood test is an important screening tool for early detection of digestive tract diseases, especially colon cancer. Understanding how it works can help correctly interpret the test results and take appropriate follow-up measures.
Features:
- Product Name: Colloidal Gold Rapid Test
- Detection Limit: High Sensitivity
- Application: Clinical/ Hospital/ Laboratory/ Home
- Package: 25/50/100 Tests/kit
- Shelf Life: 24 Months
- Type: Colloidal Gold
- Can be used as a Home Test or Self Test
Technical Parameters:
| Format | Cassette/Strip |
| Product Name | Fecal occult blood test |
| Storage Temperature | 2-30℃ |
| Detection Limit | High Sensitivity |
| Shelf Life | 24 Months |
| Type | Colloidal Gold |
| Sample Type | WB/S/P |
| Application | Clinical/ Hospital/ Laboratory/ Home |
| Reading Time | 5-15 Minutes |
| Test Method | Rapid Test |
Applications:
Routine health screenings, such as colon cancer screening.
Diagnosing the cause of gastrointestinal bleeding.
Monitoring the effectiveness of treatment for digestive disorders.
Support and Services:
The Colloidal Gold Rapid Test is a diagnostic tool designed for the qualitative detection in human fece samples. Technical support and services offered with this product include:
- Instructions for use and interpretation of results
- Technical assistance and troubleshooting
- Product training and education
- Product customization and development
- Quality control and assurance
- Regulatory compliance support
