ISO13485 HIV Ab Ag Test Hiv 4th Generation Assay ELISA Kit 60 Minutes

Hot Sale HIV Ab & Ag ELISA 96 Test/Kit
Catalog No.: BE701A
1. INTENDED USE

• This is a 4th-generation assay for the simultaneous detection of antibodies to HIV-1 and HIV 2 as well as p24 antigen of HIV-1. The assay is based on recombinant and synthetic peptides for antibody detection and monoclonal antibody specific to the p24 protein of HIV-1.
• It has great significance in the diagnosis of HIV infection / screen for donated blood to prevent transmission of HIV to recipients and as an aid in clinical diagnosis of HIV-related infections.

2. Materials provided with the kits:

3. ASSAY PROCEDURE
1. Prepare Reagents: Dilute 1 volume of Washing Buffer with 19 volumes of distilled water, mix well.

2. Add Conjugate 1 and Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2
wells as Negative Control, 2 wells as Positive Control HIV-1, 2 wells Positive Control HIV-2 and 2 wells
Positive Control P24 Antigen. After dispensing 75μL of sample or Negative Control or Positive Control to
the respective wells, dispense 25μL of Conjugate 1 to each well (except the blank well). Gently vibrating
the plate.

3. Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled
water-bath or microplate incubator at 37℃ for 60 minutes.

4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer (10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure
that the rest volume is minimal, by tapping plate onto absorbent paper.

5. Add Conjugate 2: Add 100μL of Conjugate 2 to each well (except the blank well).

6. Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.

7. Wash the Plate: Repeat the wash procedure as in step 4.

8. Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well.
Cover and incubate at 37℃ for 30 minutes.

9. Stop reaction: Add 50μL Stop Solution to each well, mix well.

10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.

60 Minutes HIV Ab Ag Test

Hiv 4th Generation Assay ELISA Kit

ISO13485 HIV Ag Ab Test

ISO13485 HIV Ab Ag Test Hiv 4th Generation Assay ELISA Kit 60 Minutes Images